Vitamin C and E Supplementation Can Mitigate NaF Mediated Hematological and Hepatic Disorders in Adult Wistar Rats

Abstract

The study aimed to investigate the effect of combined vitamin E and C, on hematological and hepatic systems of adult male Wistar rats with fluoride toxicity. Eighteen rats were randomly divided into three groups such as control, treated (given NaF, 20 mg/kg bw/rat/day), and supplemented (NaF and vitamin E-200 mg/kg bw + C- 400 mg/kg bw). Altered hematological parameters like erythrocyte count, leukocyte count and hemoglobin concentration were seen in NaF groups. Estimation of osmotic fragility of erythrocyte showed a marked increase fragile erythrocytes in fluoride-treated groups compared to control and supplemented groups. Total thiol concentration was also decreased due fluoride fluoride-induced oxidative stress and it was protected in supplementary groups. The oxidative imbalance induced by NaF in liver was evaluated by estimating the activity of antioxidant enzyme superoxide dismutase and catalase and with estimation of lipid peroxidation and protein oxidation product malondialdehyde and protein carbonyl. All enzymatic and non-enzymatic markers of oxidative imbalance were significantly altered (increased/decreased) due to fluoride toxicity, which was again observed to be protected in the supplemented group. The histopathological analysis of the liver also proved the mitigatory effect of the combined vitamin E+C on fluorideinduced hepatic insult.