Determination of LD50 and ED50 Value of Nicotine Doses in Relation to Body and Organs Weight Along with Haematological Parameter: A Day-Dependent Experimental Study on Male Albino Rats

Abstract

As we know that nicotine toxicity is accountable for several damages at cellular level. Cellular damage directly or indirectly causes unhealthy impact on body and organ weight. Still now a little research work has been conducted regarding the nicotine induced changes in body and organ weight (Heart, Liver etc)along with haematological (RBC, WBC, Platelets and hemoglobin) parameters in male albino rat. In this study we tried to find out the effects of LD50and ED50 of nicotine on above parameters. We appliedLD50 and ED50 doses of nicotine on day-dependent study (10, 15 and 20 days) and observed its effect on rat model. In this experimental study the doses of LD50 and ED50 of nicotine were 51mg/kg body weight and 3.5mg/kg body weight respectively. After 10 days of treatment, no significant changes were observed. But during 15 days of nicotine treatment, it produced toxic effects on the studied parameters. But all those parameters were more aggravated during 20 days of nicotine application. All the results were highly significant at P<0.05 level in compared to that of control group. In conclusion, it is suggested that the effective dose of nicotine of 3.5mg/kg body weight caused toxic effects in this day-dependent study.