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dc.contributor.advisorChattopadhyay, Sandipen_US
dc.contributor.advisorMaiti, Samarjiten_US
dc.contributor.authorDeb, Bimal-
dc.description.abstractThe present study elucidated the profound protective role of vitamin B and folic acid against arsenic-mediated reproductive toxicity in female rats. Ingestion of sodium-arsenite via drinking water [0.4 ppm/100 g body weight (b.wt.)/day] followed by the co-administration of vitamin B and folic acid (0.07 μg and 4.0 μg respectively/100 g b.wt./day) in Wistar rats represented a notable protection against arsenic-induced disruption of female gonadal function, ROS generation and DNA fragmentation of ovarian and uterine tissues as well as disorganization of uteroovarian histoarchitecture. However, arsenication impaired the action of the free radical scavenging enzymes superoxide dismutase (SOD) and catalase, peroxidase (POD) in ovarian and uterine tissue, with a concomitant elevation in lipid peroxidation in the reproductive organs. Arsenic ingestion resulted significant drop in the circulating follicle-stimulating hormone (FSH), leutinizing hormone (LH), and estradiol along with retarded activities of  12 5 ,3β -hydroxysteroid dehydrogenase (5,3β-HSD) and 17β-hydroxysteroid dehydrogenase (17β-HSD). Arsenicated rats showed irregular estrous cycle dominated by lengthy diestrus. Our previous work on arsenic-exposed humans was associated with DNA injury and carcinogenesis. Here, we demonstrated that the supplementation of aforesaid physiological/therapeutic dose of vitamin B and folate protected the rodents appreciably from arsenicinduced DNA damage (DNA fragmentation and comet assay) and ovarian and uterine tissue disintegration (histoarchitecture, HE staining). Vitamin supplementation mitigated the arsenic mediated reproductive injury by preventing abundant generation of free radicals. Restrained generation of free radicals may be correlated to the protection of DNA stability and reproductive organs morphology. This study highlighted that the decisive role of vitamin B 12 and folic acid in ameliorating arsenic-mediated reproductive disorder.en_US
dc.publisherVidyasagar University, Midnapore, West Bengal, India,en_US
dc.subjectDNA fragmentationen_US
dc.subjectArsenic- Drinking wateren_US
dc.subjectUtero-ovarian Histoarchitectureen_US
dc.subjectFemale Rat - Reproductive and metabolic profileen_US
dc.titleImpact of Vitamin B12 and Folic acid on Arsenic Induced Female Reproductive Status of Albino Ratsen_US
Appears in Collections:Bio-Medical Laboratory Science & Management - Ph.D

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02_certificates.pdf1.88 MBAdobe PDFView/Open
03_abstract.pdf155.08 kBAdobe PDFView/Open
04_declaration.pdf156.75 kBAdobe PDFView/Open
05_Acknowledgement.pdf7.62 kBAdobe PDFView/Open
06_contents.pdf222.91 kBAdobe PDFView/Open
07_list of tables.pdf183.48 kBAdobe PDFView/Open
08_list_of_figures.pdf209.59 kBAdobe PDFView/Open
09_abbreviations.pdf172.94 kBAdobe PDFView/Open
10_chapter 1.pdf233.78 kBAdobe PDFView/Open
11_chapter 2.pdf6.83 MBAdobe PDFView/Open
12_chapter 3.pdf133.03 kBAdobe PDFView/Open
13_chapter 4.pdf3.84 MBAdobe PDFView/Open
14_chapter 5.pdf22.53 MBAdobe PDFView/Open
15_chapter 6.pdf361.4 kBAdobe PDFView/Open
16_conclusion.pdf98.46 kBAdobe PDFView/Open
17_ summary.pdf205.89 kBAdobe PDFView/Open
18_bibliography.pdf404.27 kBAdobe PDFView/Open
List of Publications.pdf138.24 kBAdobe PDFView/Open

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