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dc.contributor.advisorChattopadhyay, Sandipen_US
dc.contributor.advisorIslam, Syed Sirajulen_US
dc.contributor.authorParveen, Hasina-
dc.description.abstractArsenic is a nonessential trace element. A large population worldwide is being affected by sodium arsenite polluted drinking water. Arsenic intoxication is responsible for the severe health hazards mainly infertility in humans and animals. Various treatment approaches: DMSA and BAL becoming a challenge to manage the arsenic-induced reproductive malfunction. Sometimes this chelation remedy for arsenic induced health hazards gives rise to moderate to severe side effects. Now a day’s researchers tried to use different natural herbal plant extracts and polymer-based nanoparticles to overcome the problem of arsenisation in animal model. Hence, the present thesis work emphasized to mature a non- invasive therapeutic model using chitosan, curcumin and pectic polysaccharide. The present study focused the structure of the extracted pectic polysaccharide (CCPS) of bitter gourd (Momordica charantia) contains D-methyl galacturonate and D-galactose in 4:1 molar ratios. FTIR study of CCPS indicates that it has hydroxyl groups to bind with arsenic in its structure. Series of negatively charged galacturonate remains in CCPS provides the better potential activity for the cation chelation. The synthesized water soluble encapsulated curcumin chitosan nanoparticles (ECNPs) having a diameter of 8-40 nanometres appeared to relief arsenic-mediated hazards. These studies fulfill to search out the efficacy of curcumin, CCPS and ECNPs against sodium-arsenite mediated female repro-toxicity. The solo or combined treatment mode at 20 mg/ Kg BW of the curcumin, 2.0 mg/ Kg BW of CCPS and 1.0 mg/ Kg BW of ECNPs doses were selected against 10 mg/ Kg BW of sodium arsenite. The curcumin, CCPS and ECNPs extensively attenuate the arsenic-mediated oxidative stress and lipid peroxidation level in uterus and ovary. Treatment of these biomolecules mitigates the suppression of ovarian steroidogenesis in the arsenicated Wistar rats by regulating the estradiol receptor along with the normal tissue histo-architecture. Oral administration of curcumin and CCPS also suppress the inflammatory markers TNF-α, IL-6, and NF-қB in the arsenicated rats. Up-regulation of Bax, caspase-3, PARP, PCNA and phospho p53 in arsenicated rats was followed by down regulation of Bcl 2 and AKT respectively. CCPS significantly reverts back these arsenic induced expressional changes. Dietary CCPS also makes sure the successful fertility rate with healthy pups in arsenic fed rats. This study helps to understand the underlying mechanism of curcumin, CCPS and ECNPs in attenuating arsenic mediated uterine and ovarian dysfunction involving regulation of SAM pool components, B12 , folate and homocysteine. Moreover, the encapsulated curcumin-chitosan nanoparticles at tiny establish greater efficacy than that of higher dose of curcumin alone against the arsenic induced female repro-toxicity.en_US
dc.publisherVidyasagar University, Midnapore, West Bengal, India,en_US
dc.subjectFemale Reproductive Toxicityen_US
dc.subjectOxidative Stressen_US
dc.subjectPolysaccharide (CCPS)en_US
dc.subjectCurcumin encapsulated Chitosan Nanoparticles (ECNPs)en_US
dc.titleAnalysis of therapeutic strength of Curcumin, Chitosan and other Polysaccharides for the management of arsenic induced Ovarian and Uterine Anarchyen_US
Appears in Collections:Bio-Medical Laboratory Science & Management - Ph.D

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02_certificate.pdf238.2 kBAdobe PDFView/Open
03_abstract.pdf75.79 kBAdobe PDFView/Open
04_declaration.pdf128.24 kBAdobe PDFView/Open
05_Acknowledgement.pdf138.65 kBAdobe PDFView/Open
06_contents.pdf160.44 kBAdobe PDFView/Open
07_list_of_tables.pdf49.48 kBAdobe PDFView/Open
08_list_of_figures.pdf114.3 kBAdobe PDFView/Open
09_abbreviations.pdf88.22 kBAdobe PDFView/Open
10_chapter1.pdf133.79 kBAdobe PDFView/Open
11_chapter2.pdf88.38 kBAdobe PDFView/Open
12_chapter3.pdf157.95 kBAdobe PDFView/Open
13_chapter4.pdf229.84 kBAdobe PDFView/Open
14_chapter5.pdf278.2 kBAdobe PDFView/Open
15_chapter6.pdf567.39 kBAdobe PDFView/Open
16_chapter7.pdf298.29 kBAdobe PDFView/Open
17_chapter8.pdf312.57 kBAdobe PDFView/Open
18_chapter9.pdf1.51 MBAdobe PDFView/Open
19_chapter10.pdf1.14 MBAdobe PDFView/Open
20_chapter11.pdf309.95 kBAdobe PDFView/Open
21_chapter12.pdf345.04 kBAdobe PDFView/Open
22_conclusion13.pdf88.59 kBAdobe PDFView/Open
23_summary14.pdf72.14 kBAdobe PDFView/Open
24_bibliography15.pdf254.5 kBAdobe PDFView/Open
25_list_of_publications16.pdf66.54 kBAdobe PDFView/Open

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