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http://inet.vidyasagar.ac.in:8080/jspui/handle/123456789/1314
2024-03-28T15:10:19ZConsequence of dietary n-Acetyl Cysteine on Arsenic mediated female reproductive hazards
http://inet.vidyasagar.ac.in:8080/jspui/handle/123456789/6310
Title: Consequence of dietary n-Acetyl Cysteine on Arsenic mediated female reproductive hazards
Authors: Dash, Moumita
Abstract: Background: Arsenic contamination consistently provides a negative impact
especially by drinking water and resulted in many fatal disorders. Numerous
treatment approaches have been availed to combat arsenic assisted distress with
plenty of side effects. Antioxidants with chelation properties become more
advantageous to treat arsenication with the least toxicity.
Aims & objectives: Present study highlighted the expansion of risk-free, safe, and
nontoxic treatment strategy to oppose arsenication. The whole experimentation also
focused on the antioxidant plus chelating possession and remedial index of NAC
against sodium arsenite driven reproductive complications in rat model.
Methods: In different mode of treatment sodium arsenite and NAC were employed
wherein 10 mg/kg body weight of sodium arsenite and 100 mg/kg body weight of
NAC were applied respectively. But the dietary application of NAC was provided at
the dose of 250 mg/kg body weight. Arsenic mediated oxidative stress was justified
by the assay of MDA-CD, SOD, catalase plus GPx activities along with NPSH.
Arsenic interfered usual reproductive functions were justified by the assay of ∆
HSD and 17β-HSD, and also ovarian hormones like LH, estradiol, and FSH were
studying the regulation of reproductive functions of female rats. Serum SGOT,
SGPT, creatinine were assayed to indicate hepato-renal functionality. Few
inflammatory indicators were assayed such as TNF-α, IL-6, NF-B and also
apoptotic markers (Bax, Bcl-2, p53) were assessed. Serum level of vitamin B
I, folic acid, homocysteine were assayed as well. DNA damage, histopathology of
12
5
, 3β-
, MT-ovarian-uterine tissues, immunohistochemistry for affirmation of ER-α was also
illustrated.
Results: Arsenic aggravated oxidative stress implicated with higher levels of MDA-
CD with lowering activity of antioxidative enzymes. Steroidogenic action in ovary
was suppressed notably by diminishing the level of gonadotropins and estradiol in
circulation. This causes structural dysintegration of ovarian-uterine tissues. Arsenic
also promulgated DNA abnormalities by higher and significant discharge of
inflammatory indicators and stimulating the pro-apoptotic gene manifestation. The
controlling function of NAC worked against all these abnormal circumstances and
brought back in homeostasis. Arsenic primed over-creation of oxidative stress was
neutralized by NAC via improving antioxidant enzymatic activity in repro-organs.
NAC guided improvement of gonadotropin’s utility significantly favoured ovarian
steroidogenesis. The inflammatory condition following arsenication was well
managed by the accessibility of NAC and also controlled the apoptotic progression.
The important vitamin’s level was up-surged in NAC availed group while protected
against homocysteine surge in arsenite challenged group.
Conclusion: The above said outcomes specified that NAC may be a novel bio-
component against arsenic and its proposed adverse implications. Antioxidant plus
chelating conformity of NAC combat against arsenic directed oxidative stress. Also
it has anti-apoptotic and anti-inflammatory possessions and thus maintains the
system from arsenic aggravated complications.2021-10-06T00:00:00ZImpact of Vitamin B12 and Folic acid on Arsenic Induced Female Reproductive Status of Albino Rats
http://inet.vidyasagar.ac.in:8080/jspui/handle/123456789/6240
Title: Impact of Vitamin B12 and Folic acid on Arsenic Induced Female Reproductive Status of Albino Rats
Authors: Deb, Bimal
Abstract: The present study elucidated the profound protective role of vitamin B
and folic
acid against arsenic-mediated reproductive toxicity in female rats. Ingestion of
sodium-arsenite via drinking water [0.4 ppm/100 g body weight (b.wt.)/day]
followed by the co-administration of vitamin B
and folic acid (0.07 μg and 4.0 μg
respectively/100 g b.wt./day) in Wistar rats represented a notable protection against
arsenic-induced disruption of female gonadal function, ROS generation and DNA
fragmentation of ovarian and uterine tissues as well as disorganization of uteroovarian
histoarchitecture. However, arsenication impaired the action of the free
radical scavenging enzymes superoxide dismutase (SOD) and catalase, peroxidase
(POD) in ovarian and uterine tissue, with a concomitant elevation in lipid
peroxidation in the reproductive organs. Arsenic ingestion resulted significant drop
in the circulating follicle-stimulating hormone (FSH), leutinizing hormone (LH),
and estradiol along with retarded activities of
12
5
,3β -hydroxysteroid dehydrogenase
(5,3β-HSD) and 17β-hydroxysteroid dehydrogenase (17β-HSD). Arsenicated rats
showed irregular estrous cycle dominated by lengthy diestrus. Our previous work on
arsenic-exposed humans was associated with DNA injury and carcinogenesis. Here,
we demonstrated that the supplementation of aforesaid physiological/therapeutic
dose of vitamin B
and folate protected the rodents appreciably from arsenicinduced
DNA damage (DNA fragmentation and comet assay) and ovarian and
uterine tissue disintegration (histoarchitecture, HE staining). Vitamin
supplementation mitigated the arsenic mediated reproductive injury by preventing
abundant generation of free radicals. Restrained generation of free radicals may be
correlated to the protection of DNA stability and reproductive organs morphology.
This study highlighted that the decisive role of vitamin B
12
and folic acid in
ameliorating arsenic-mediated reproductive disorder.2021-08-02T00:00:00ZRole of Arjunolic acid and vitamin B12 on Arsenic induced Ovarian Malfunction: An approach through the study of S-Adenosine Methionine Pool
http://inet.vidyasagar.ac.in:8080/jspui/handle/123456789/6059
Title: Role of Arjunolic acid and vitamin B12 on Arsenic induced Ovarian Malfunction: An approach through the study of S-Adenosine Methionine Pool
Authors: Maity, Moulima
Abstract: People of wide area of India are slowly poisoned through the arsenic contaminated drinking
water. The affected people of low economic groups from rural area are suffering with skin
cancer, metabolic disorders, reproductive hazards, infertility etc. It is global challenge to prevent
arsenic induced reproductive toxicity. There are very limited chelating agents found in the
market (BAL, DMSA, DMPS) to prevent arsenic induced health hazards but these have
noninvasive and painful treatment strategy. Hence, we have planned to develop an easily
available cost-effective drug against arsenic toxicity which has no side effects. To fulfill this
endeavor we have chosen arjunolic acid and vitamin B12 to observe the effects against arsenic
induced female repro-toxicity. Sodium arsenite (1.0mg/100gm body weight) was given to the
adult female Wistar strain rats with arjunolic acid (1.0mg/100gm body weight) and vitamin B12
(0.09μg/100gm body weight) alone or in combination to find out preventive, protective and
curative effect of these two. Arjunolic acid and vitamin B12 could prevent arsenic induced ROS-
production through the alteration of the antioxidants activities, ovarian steroidogenesis and
inflammatory response in preventive, protective and curative manner as focused from in vivo
experimental model. These mitigating effects may involve an indirect mechanism associated
with a critical role of hypothalamico-pituitary-ovarian axis, although arjunolic acid and B12 had
shown its ability to exert its effect directly on the reproductive organs as revealed from the
experiment on in vitro model.2021-04-18T00:00:00ZAnalysis of therapeutic strength of Curcumin, Chitosan and other Polysaccharides for the management of arsenic induced Ovarian and Uterine Anarchy
http://inet.vidyasagar.ac.in:8080/jspui/handle/123456789/5738
Title: Analysis of therapeutic strength of Curcumin, Chitosan and other Polysaccharides for the management of arsenic induced Ovarian and Uterine Anarchy
Authors: Parveen, Hasina
Abstract: Arsenic is a nonessential trace element. A large population worldwide is being affected by
sodium arsenite polluted drinking water. Arsenic intoxication is responsible for the severe
health hazards mainly infertility in humans and animals. Various treatment approaches:
DMSA and BAL becoming a challenge to manage the arsenic-induced reproductive
malfunction. Sometimes this chelation remedy for arsenic induced health hazards gives rise
to moderate to severe side effects. Now a day’s researchers tried to use different natural
herbal plant extracts and polymer-based nanoparticles to overcome the problem of
arsenisation in animal model. Hence, the present thesis work emphasized to mature a non-
invasive therapeutic model using chitosan, curcumin and pectic polysaccharide. The present
study focused the structure of the extracted pectic polysaccharide (CCPS) of bitter gourd
(Momordica charantia) contains D-methyl galacturonate and D-galactose in 4:1 molar ratios.
FTIR study of CCPS indicates that it has hydroxyl groups to bind with arsenic in its structure.
Series of negatively charged galacturonate remains in CCPS provides the better potential
activity for the cation chelation. The synthesized water soluble encapsulated curcumin
chitosan nanoparticles (ECNPs) having a diameter of 8-40 nanometres appeared to relief
arsenic-mediated hazards. These studies fulfill to search out the efficacy of curcumin, CCPS
and ECNPs against sodium-arsenite mediated female repro-toxicity. The solo or combined
treatment mode at 20 mg/ Kg BW of the curcumin, 2.0 mg/ Kg BW of CCPS and 1.0 mg/ Kg
BW of ECNPs doses were selected against 10 mg/ Kg BW of sodium arsenite. The curcumin,
CCPS and ECNPs extensively attenuate the arsenic-mediated oxidative stress and lipid
peroxidation level in uterus and ovary. Treatment of these biomolecules mitigates the
suppression of ovarian steroidogenesis in the arsenicated Wistar rats by regulating the
estradiol receptor along with the normal tissue histo-architecture. Oral administration of
curcumin and CCPS also suppress the inflammatory markers TNF-α, IL-6, and NF-қB in the arsenicated rats. Up-regulation of Bax, caspase-3, PARP, PCNA and phospho p53 in
arsenicated rats was followed by down regulation of Bcl
2
and AKT respectively. CCPS
significantly reverts back these arsenic induced expressional changes. Dietary CCPS also
makes sure the successful fertility rate with healthy pups in arsenic fed rats. This study helps
to understand the underlying mechanism of curcumin, CCPS and ECNPs in attenuating
arsenic mediated uterine and ovarian dysfunction involving regulation of SAM pool
components, B12 , folate and homocysteine. Moreover, the encapsulated curcumin-chitosan
nanoparticles at tiny establish greater efficacy than that of higher dose of curcumin alone
against the arsenic induced female repro-toxicity.2021-01-23T00:00:00Z